cDNA Microarray Gene Expression Analysis and Toxicological Phenotype for Anticancer Drug

cDNA MICROARRAY TECHNOLOGY FOR THE ANALYSIS OF GENE EXPRESSION

DNA microarray technology is a new and powerful tool for studying a genome-wide gene expression in many organisms. This technology is a high throughput, highly parallel RNA expression assay technique that permits quantitative analysis of RNAs transcribed from both known and unknown genes. This review describes array manufacturing, experimental design, and its application to gene discovery and e...

Assessment of gene expression profile by cDNA microarray analysis.

With the advent of cDNA microarray technology, variations in cellular transcriptional events can now be monitored simultaneously, and expression profile at each functional state can be established and compared to one another. Here we illustrate gene expression profile analysis of a cisplatin-resistant ovarian carcinoma cell line (A2780CP)and its parental A2780 cell line, and compare them to ass...

cDNA Microarray Data Normalization

Hierarchical Bayes models for cDNA microarray gene expression.

cDNA microarrays are used in many contexts to compare mRNA levels between samples of cells. Microarray experiments typically give us expression measurements on 1000-20 000 genes, but with few replicates for each gene. Traditional methods using means and standard deviations to detect differential expression are not satisfactory in this context. A handful of alternative statistics have been devel...

Identification of specific gene expression after exposure to low dose ionizing radiation revealed through integrative analysis of cDNA microarray data and the interactome

Background: Accumulating reports suggest that the biological effects of low- and high- dose ionizing radiation (LDIR and HDIR) are qualitatively different and might cause different effects in human skin. Materials and Methods: To better understand the potential risks of LDIR, we analyzed three cDNA microarray datasets from the Gene Expression Omnibus database. Results: A pathway analysis showed...